Effects of astilbin on the expression of TNF alpha and IL-10 in liver warm ischemia-reperfusion injury / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 463-466, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-326334
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the effects of astilbin on the expressions of TNF alpha and IL-10 during liver warm ischemia-reperfusion injury.</p><p><b>METHODS</b>C57BL/ 6 mice were randomly divided into 4 groups (n = 8) sham-operated group (Sham), model control group(I/R), low dosage of astilbin treatment group (10 mg/kg) and high dosage of astilbin (40 mg/kg) treatment group. The treatment group mice were intraperitoneally injected with 10 or 40 mg/kg astilbin 24 hours and one hour before Ischemia, the hepatic ischemia-reperfusion model were thus established. After jn90 of min ischemia and 6 h reperfusion of the partial hepatic lobe, the expressions of TNF alpha and IL-10 in liver tissues collected from the experimental groups were detected by Western blot and semiquantitative RT-PCR.</p><p><b>RESULTS</b>The expression of TNF alpha protein in liver tissues gradually decreased in treatment groups (low and high dosages of astilbin treatment groups) as compared to the I/R model control group. Similar results were observed in the mRNA expressions of these genes as determined by semiquantitative RT-PCR (P less than 0.05 for low dosage group; P less than 0.01 for high dosage group). Compared with the I/R model control group, the expression of IL-10 was increased in both treatment groups (low dosage group P less than 0.05; large dosage group P less than 0.01).</p><p><b>CONCLUSION</b>Treatment with astilbin decreases TNF alpha expression but induces IL-10 expression in liver during warm ischemia-reperfusion injury.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Daño por Reperfusión
/
Factor de Necrosis Tumoral alfa
/
Interleucina-10
/
Flavonoles
/
Isquemia Tibia
/
Hígado
/
Metabolismo
/
Ratones Endogámicos C57BL
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Hepatology
Año:
2010
Tipo del documento:
Artículo
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