The effect of CD137-CD137 ligand interaction on the expression of nuclear factor of activated T cells c1 in apolipoprotein E-deficient mice atherosclerotic plaque model / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 775-779, 2012.
Article
en Zh
| WPRIM
| ID: wpr-326422
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of CD137-CD137L interaction on the nuclear factor of activated T cells c1 (NFATc1) in apolipoprotein E knockout (ApoE(-/-)) mice.</p><p><b>METHODS</b>Atherosclerotic plaque model was produced by rapid perivascular carotid collar placement in ApoE(-/-) mice. In vivo, the expression of NFATc1 in mice plaque and lymphocytes was detected by immunohistochemical and flow cytometry, respectively. In vitro, the NFATc1 mRNA and protein expressions in cultured lymphocytes of ApoE(-/-) mice were measured by RT-PCR and flow cytometry, respectively.</p><p><b>RESULTS</b>In vivo, after stimulating CD137-CD137L signal pathway, the expression of NFATc1 was significantly upregulated in the atherosclerotic plaques and lymphocytes. In vitro, the mRNA and protein expressions of NFATc1 in cultured leukocytes of ApoE(-/-) mice were also significantly increased, the maximal effect appeared post 20 µg/ml anti-CD137 mAb-stimulation and reached maximum at 24 h at any concentrations. Anti-CD137L mAb significantly downregulated the mRNA and protein expressions of NFATc1 in lymphocytes of ApoE(-/-) mice, maximal effect appeared at 20 µg/ml anti-CD137L mAb and reached minimum at 24 h.</p><p><b>CONCLUSION</b>CD137-CD137L interactions can modulate the expression of NFATc1 in this ApoE(-/-) mice atherosclerotic plaque model.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Apolipoproteínas E
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ARN Mensajero
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Ratones Noqueados
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Modelos Animales de Enfermedad
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Factores de Transcripción NFATC
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Ligando 4-1BB
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Miembro 9 de la Superfamilia de Receptores de Factores de Necrosis Tumoral
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Placa Aterosclerótica
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Genética
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Metabolismo
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Año:
2012
Tipo del documento:
Article