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The effect of CD137-CD137 ligand interaction on the expression of nuclear factor of activated T cells c1 in apolipoprotein E-deficient mice atherosclerotic plaque model / 中华心血管病杂志
Chinese Journal of Cardiology ; (12): 775-779, 2012.
Artículo en Chino | WPRIM | ID: wpr-326422
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of CD137-CD137L interaction on the nuclear factor of activated T cells c1 (NFATc1) in apolipoprotein E knockout (ApoE(-/-)) mice.</p><p><b>METHODS</b>Atherosclerotic plaque model was produced by rapid perivascular carotid collar placement in ApoE(-/-) mice. In vivo, the expression of NFATc1 in mice plaque and lymphocytes was detected by immunohistochemical and flow cytometry, respectively. In vitro, the NFATc1 mRNA and protein expressions in cultured lymphocytes of ApoE(-/-) mice were measured by RT-PCR and flow cytometry, respectively.</p><p><b>RESULTS</b>In vivo, after stimulating CD137-CD137L signal pathway, the expression of NFATc1 was significantly upregulated in the atherosclerotic plaques and lymphocytes. In vitro, the mRNA and protein expressions of NFATc1 in cultured leukocytes of ApoE(-/-) mice were also significantly increased, the maximal effect appeared post 20 µg/ml anti-CD137 mAb-stimulation and reached maximum at 24 h at any concentrations. Anti-CD137L mAb significantly downregulated the mRNA and protein expressions of NFATc1 in lymphocytes of ApoE(-/-) mice, maximal effect appeared at 20 µg/ml anti-CD137L mAb and reached minimum at 24 h.</p><p><b>CONCLUSION</b>CD137-CD137L interactions can modulate the expression of NFATc1 in this ApoE(-/-) mice atherosclerotic plaque model.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Apolipoproteínas E / ARN Mensajero / Ratones Noqueados / Modelos Animales de Enfermedad / Factores de Transcripción NFATC / Ligando 4-1BB / Miembro 9 de la Superfamilia de Receptores de Factores de Necrosis Tumoral / Placa Aterosclerótica / Genética / Metabolismo Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Cardiology Año: 2012 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Apolipoproteínas E / ARN Mensajero / Ratones Noqueados / Modelos Animales de Enfermedad / Factores de Transcripción NFATC / Ligando 4-1BB / Miembro 9 de la Superfamilia de Receptores de Factores de Necrosis Tumoral / Placa Aterosclerótica / Genética / Metabolismo Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Cardiology Año: 2012 Tipo del documento: Artículo