Chromatin immunoprecipitation and its preliminary application for gene regulation / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To verify the binding of p53 to p21WAF1/CIP1 gene promoter and detect its binding to hsp90 beta gene promoter in vivo.</p><p><b>METHODS</b>Chromatin immunoprecipitation and PCR analysis were used to measure specific gene regulation sequence and Western blot analysis to investigate p53 protein.</p><p><b>RESULTS</b>The p53 binding sequences on the promoters of p21WAF1/CIP1 and hsp90 beta gene were found in the p53 antibody immunoprecipitated DNA fragments and p53 was detected in the immunoprecipitated samples.</p><p><b>CONCLUSIONS</b>p53 binds to promoters of p21WAF1/CIP1 and hsp90 beta gene in vivo, and regulates the expression of the two genes.</p>