Evaluation of enzymatic method for determination of serum beta-hydroxybutyrate and its clinical application / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 702-705, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-327004
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate an enzymatic method for determining serum beta-hydroxybutyrate (beta-HB) with the National Committee for Clinical Laboratory Standards (NCCLS) projects, and to discuss its clinical values in diabetic ketoacidosis (DKA).</p><p><b>METHODS</b>The precision, accuracy, specificity, linearity and interference of the enzymatic method were analyzed. This method was used to determine serum beta-HB in 60 cases of normals, 50 cases of diabetes, and 34 cases of DKA by autochemistry analyzer.</p><p><b>RESULTS</b>Enzymatic beta-HB assay was precise (within-run CV, day-to-day CV, and total CV < 5%). The linearity studies showed the method was linear up to 4 mmol/L. Recovery rate was 98.5%-104.1%. Hemolysis (Hemoglobin up to 18.2 g/L), icteric samples with total bilirubin up to 224 mumol/L, and lipemia up to triglyceride concentration of 2.28 mmol/L did not interfere with the beta-HB results in this method. Serum beta-HB levels were significantly elevated in DKA patients compared with DM patients and controls (P < 0.01). Positive rate of serum beta-HB in DKA patients was significantly higher than that of urinary ketone (P < 0.05).</p><p><b>CONCLUSIONS</b>Enzymatic method is convenient and reliable, allows full automation, and is rapid enough to be used for both routine and urgent determinations of serum beta-HB. It can be used in diagnosing and monitoring treatment of DKA.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Autoanálisis
/
Sangre
/
Cetoacidosis Diabética
/
Ácido 3-Hidroxibutírico
/
Diabetes Mellitus
/
Estudios de Evaluación como Asunto
Tipo de estudio:
Estudio diagnóstico
/
Estudios de evaluación
/
Guía de Práctica Clínica
Límite:
Adolescente
/
Adulto
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2003
Tipo del documento:
Artículo
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