Preparation and performance of Chitosan-oligosaccharides/DNA complex nanoparticles / 生物工程学报
Chinese Journal of Biotechnology
; (12): 741-745, 2007.
Article
en Zh
| WPRIM
| ID: wpr-327954
Biblioteca responsable:
WPRO
ABSTRACT
Comparing to Chitosan, Chitosan-oligosaccharides have several special functions, such as water-soluble, antitumor activity, immunostimulating effects, and antimicrobial activity. The chitosan-oligosaccharide, the molecular weight of which was about 5000, was used as research model. According to the agarose gel electrophoresis and UV spectrophotometer it was proved that electrostatic interaction was playing a very important role in the formation process of chitosan-oligosaccharide/DNA complex. The potential of adsorbing DNA on chitosan-oligosaccharide was analyzed by gel electrophoresis and UV spectrophotometer, and it was indicated that chitosan-oligosaccharide can improve the storage and structure stability of DNA. To check its protection ability to DNA by DNase I digestive experiment, the result showed that chitosan-oligosaccharide could load with plasmid effectively and protect DNA from being digested by DNase I. It was proved that chitosan-oligosacchide was safe and effective for gene delivery and will have a very good future in the field of gene therapy.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Oligosacáridos
/
ADN
/
Química
/
Técnicas de Transferencia de Gen
/
Quitosano
/
Nanopartículas
/
Vectores Genéticos
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2007
Tipo del documento:
Article