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Construction and expression of a novel bisbicistronic expression vector: pCMV-Myc-IRES-EGFP / 生物工程学报
Chinese Journal of Biotechnology ; (12): 423-428, 2007.
Artículo en Chino | WPRIM | ID: wpr-328011
ABSTRACT
It is often necessary to construct more than one recombinant plasmids when investigating the characteristics, physchemical features and functional mechanisms of genes or proteins. Repeated sub-cloning procedures including design of primers, enzyme digestion, ligation and verification of recombinant plasmids, have to be involved with. For this reason, it has become a tendency to developing new genetic vectors which can be used in multitude of experiments. Therefore, by using pIRES vector as a backbone, here we reported the construction of a mammalian expression vector pCMV-Myc-IRES-EGFP which contains the N-terminal c-Myc epitope tag and the enhanced green fluorescent protein (EGFP) translated in an IRES-dependent manner. This novel vector can be used to testify the efficiency of cell transfection, to collect successfully transfected cell population via cytometry, to conduct transcription and translation in vitro, to purify target proteins or to trap their interactional proteins. The availability of this vector can facilitate function study of genes.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Recombinantes de Fusión / Datos de Secuencia Molecular / Secuencia de Bases / Transfección / Expresión Génica / Línea Celular / Western Blotting / Genes myc / Clonación Molecular / Proteínas Fluorescentes Verdes Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Proteínas Recombinantes de Fusión / Datos de Secuencia Molecular / Secuencia de Bases / Transfección / Expresión Génica / Línea Celular / Western Blotting / Genes myc / Clonación Molecular / Proteínas Fluorescentes Verdes Límite: Humanos Idioma: Chino Revista: Chinese Journal of Biotechnology Año: 2007 Tipo del documento: Artículo