Establishment of a cell-based high-throughput screening model for PPARdelta agonists / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 343-346, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-328026
ABSTRACT
To establish a new high-throughput screening model for the agonist of PPARdelta, PPARdelta gene was obtained by reverse transcriptase-polymerase chain reaction (RT-PCR), and subcloned to pGEM-T Vector for sequencing, then the PPARdelta fragment was excised by restriction enzymes, and inserted into pTARGET Vector to construct expression vector pTARGET-ppARdelta. Insert three copies of PPRE into pGl3-promoter vector to construct expression vector pGl3-PPRE x 3-luc. The vector pTARGET-ppARdelta was transiently cotransfected with pGl3-PPRE x 3-luc into different cell lines to assay the expression levels of luciferase. The PPARdelta agonist screening model was established and optimized. Bezafibrate and linoleic acid can induce the expression of luciferase significantly and in a dose-dependent manner. This method can be used for high throughput screening for the agonist of PPARdelta, which might become lead compounds for new anti-atheroscleriosis or anti-adiposity drugs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Bezafibrato
/
Proteínas Recombinantes de Fusión
/
Células HeLa
/
Transfección
/
Línea Celular
/
Química
/
Células 3T3
/
Ácido Linoleico
/
PPAR delta
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2007
Tipo del documento:
Artículo
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