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FRIL maintains quiescence of hematopoietic stem cells through regulation of cell cycle related factors / 中华血液学杂志
Chinese Journal of Hematology ; (12): 37-40, 2007.
Artículo en Chino | WPRIM | ID: wpr-328374
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of Flt3 receptor-interacting lectin (FRIL) maintains quiescence of hematopoietic stem cells (HSCs) in vitro.</p><p><b>METHODS</b>Cord blood CD34+ cells were cultured in suspension medium supplemented with or without FRIL and FL. Cells were collected at different time points and the expression of some cell cycle regulators, especially those involved in G0/G1 phase regulation were detected on mRNA and protein level.</p><p><b>RESULTS</b>The expressions of G0/G1 phase related cyclins or CDKs were undetectable in the newly isolated CD34+ cells, expressions of Cyclin D3, CDK6 and P27 were the lowest in FRIL cultured group after 3d's culture (FRIL group 483 +/- 63, 553 +/- 39, 0.312 +/- 0.030; FL group 2437 +/- 52, 3209 +/- 98, 0.787 +/- 0.024; BLANK 914 +/- 105, 1497 +/- 55, 0.616 +/- 0.029, respectively), but the expression of P53 was the highest in FRIL group (FRIL group 4.476 +/- 0.159; FL group 0.581 +/- 0.099, BLANK 2.167 +/- 0.114). The expression of positive regulators of cell cycle in FRIL group were the same as that of FL group and blank group or lower.</p><p><b>CONCLUSION</b>FRIL preserves HSCs effectively in vitro through the mechanisms of down-regulation of cyclin D3 and CDK6 and activation of P53. P27 is mostly involved in the differentiation of HSCs.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / ARN Mensajero / Células Madre Hematopoyéticas / Ciclo Celular / Células Cultivadas / Proteínas de Ciclo Celular / Antígenos CD34 / Biología Celular / Lectinas de Unión a Manosa / Lectinas de Plantas Límite: Humanos Idioma: Chino Revista: Chinese Journal of Hematology Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / ARN Mensajero / Células Madre Hematopoyéticas / Ciclo Celular / Células Cultivadas / Proteínas de Ciclo Celular / Antígenos CD34 / Biología Celular / Lectinas de Unión a Manosa / Lectinas de Plantas Límite: Humanos Idioma: Chino Revista: Chinese Journal of Hematology Año: 2007 Tipo del documento: Artículo