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Alterations of CD4+ CD25+ regulatory T cells in patients with idiopathic thrombocytopenic purpura / 中华血液学杂志
Chinese Journal of Hematology ; (12): 184-188, 2007.
Artículo en Chino | WPRIM | ID: wpr-328387
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of CD4+ CD25+ regulatory T (Treg) cells in patients with ITP.</p><p><b>METHODS</b>Flow cytometry was used to examine the number of CD4+ CD25+, CD4+ CD25 high, CD4+ Foxp3+ and CD4+ CD25+ Foxp3+ T cells. The level of Foxp3 mRNA expression was analyzed by realtime quantitative reverse transcriptase polymerase chain reaction (RT-PCR) . CD4+ CD25 high T cells was cocultured with CD4+ CD25 - T cells from patients or controls for assessing the regulatory properties of CD4+ CD25 Treg cells.</p><p><b>RESULTS</b>The proportion of CD4+ CD25+ T cells in the peripheral blood of patients with ITP [(15.64 +/- 5.82) %] was significantly higher than that in normal control group [(9.30 +/- 3.95)%] (P <0.01). There was no significant difference in the percentages of CD4+ CD25 high T cells between ITP patients and controls [(1.53 +/- 0.66)% versus (1.36 +/- 0.55)% (P = 0.317)]. But the number of CD4 Foxp 3+ T cells and CD4 + CD25+ Foxp3+ T cells in patients were significantly lower than that in control group (P <0.01). The expression of Foxp3 mRNA reduced (P < 0.01) and the suppressive activity of CD4+ CD25 high T cells is lower than that of healthy controls (P <0.01).</p><p><b>CONCLUSION</b>In patients with ITP, both the number and immuno-regulative function of CD4+ CD25+ Treg cells are reduced.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ARN Mensajero / Púrpura Trombocitopénica Idiopática / Linfocitos T Reguladores / Alergia e Inmunología / Factores de Transcripción Forkhead / Subunidad alfa del Receptor de Interleucina-2 / Metabolismo Límite: Adolescente / Adulto / Anciano / Femenino / Humanos / Masculino Idioma: Chino Revista: Chinese Journal of Hematology Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ARN Mensajero / Púrpura Trombocitopénica Idiopática / Linfocitos T Reguladores / Alergia e Inmunología / Factores de Transcripción Forkhead / Subunidad alfa del Receptor de Interleucina-2 / Metabolismo Límite: Adolescente / Adulto / Anciano / Femenino / Humanos / Masculino Idioma: Chino Revista: Chinese Journal of Hematology Año: 2007 Tipo del documento: Artículo