Effects of andrographolide on the activation of mitogen activated protein kinases and nuclear factor-κB in mouse peritoneal macrophage-derived foam cells / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 391-394, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-328508
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of andrographolide on the activation of mitogen-activated protein kinases (MAPKs) and expression of nuclear factor-κB (NF-κB) in macrophage foam cells.</p><p><b>METHODS</b>The mouse peritoneal macrophages were cultured in the media in the presence of oxidized low-density lipoprotein (ox-LDL), ox-LDL+andrographolide, or neither (control). The phosphorylation of MAPK molecules (p38MAPK, JNK, ERK1/2) and the expressions of NK-κB p65 were examined by Western blot.</p><p><b>RESULTS</b>As compared with cells in the control group, the expressions of phospho-p38 and NF-κB p65 were increased in the cells cultured with either ox-LDL or ox-LDL+andrographolide (P<0.01), but attenuated significantly in the presence of ox-LDL+ andrographolide when compared with ox-LDL (P<0.05). The phospho-JNK increased in the presence of either ox-LDL or ox-LDL+andrographolide when compared with control cells (P<0.01), but no significant difference existed between ox-LDL and ox-LDL+andrographolide (P>0.05). The expression of phospho-ERK1/2 was increased in the presence of ox-LDL compared with the control cells (P<0.01), but no significant differences existed between the cells cultured in the presence of ox-LDL+andrographolide and the control medium (P>0.05).</p><p><b>CONCLUSIONS</b>Andrographolide could inhibit the activation of ERK1/2, p38MAPK and NK-κB induced by ox-LDL in macrophage foam cells, which might be one of its mechanisms in preventing atherosclerosis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Vasculitis
/
Células Cultivadas
/
FN-kappa B
/
Macrófagos Peritoneales
/
Sistema de Señalización de MAP Quinasas
/
Biología Celular
/
Quinasas MAP Reguladas por Señal Extracelular
/
Proteínas Quinasas JNK Activadas por Mitógenos
/
Proteínas Quinasas p38 Activadas por Mitógenos
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese journal of integrative medicine
Año:
2012
Tipo del documento:
Artículo
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