Effect of artemisinin combined with glucocorticoid on the expressions of glucocorticoid receptor α mRNA, glucocorticoid receptor β mRNA and P300/CBP protein in lupus nephritis mice / 中国结合医学杂志
Chinese journal of integrative medicine
;
(12): 277-282, 2011.
Artículo
en Inglés
| WPRIM
| ID: wpr-328518
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the therapeutic effects and mechanisms of using artemisinin (Art) combined with glucocorticoid (GC) to treat lupus nephritis (LN) mice.</p><p><b>METHODS</b>Forty hybrid female mice were randomly and equally divided into four groups with the method of random number table control group, model group, prednisone group administrated with 6.45 mg/(kg·d) prednisone suspension, and Art+prednisone group administrated with 150 mg/(kg·d) Art suspension and 3.225 mg/(kg·d) prednisone suspension. A mice model of LN was established by injection with living lymph cell suspension. The changes of urine protein/24h, the expressions of GC receptor α (GRα) mRNA, GC receptor β (GRβ) mRNA in peripheral blood mononuclear cells (PBMCs), and transcriptional coactivator P300/CBP protein in renal tissue were measured.</p><p><b>RESULTS</b>Compared with the model group, the treatment groups had significant decrease in urine protein/24 h, and renal pathological lesion (P<0.01). In the same groups, the expression of transcriptional coactivator P300/CBP protein in renal tissue and GRα mRNA were significantly increased, and GRβ mRNA expression was significantly decreased (P<0.01). And the Art+prednisone group has a better therapeutic effect than the prednisone group (P<0.01).</p><p><b>CONCLUSIONS</b>Art has therapeutic sensitization effects on GC in the LN mice. The underlying mechanism could be correlated with the effect of Art on the increase of the expressions of GRα mRNA and transcriptional coactivator P300 300/CBP protein in renal tissue and on the decrease of the expression of GRβ mRNA in PBMC.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Nefritis Lúpica
/
ARN Mensajero
/
Prednisona
/
Secuencia de Bases
/
Receptores de Glucocorticoides
/
Cartilla de ADN
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Artemisininas
/
Modelos Animales de Enfermedad
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese journal of integrative medicine
Año:
2011
Tipo del documento:
Artículo
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