Establishment of Chinese hamster ovary cell line expressing recombinant GPIb-IX complex / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1560-1563, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-328600
ABSTRACT
The aim of this study was to construct Chinese Hamster Ovary (CHO) cell models expressing recombinant wild-type GPIb-IX and mutant GPIb-IX complex, so as to provide the platform to study the related physiologic functions of GPIb-IX. The plasmids were extracted from E.coli expressing wild-type or deletion mutant GPIbalpha and were identified by digestion with EcoR I. Three plasmids containing GPIbalpha, GPIbbeta, and GPIX genes were co-transfected into CHO cells, and then the expression of GPIb-IX complex was detected by immune coprecipitation, Western blot and flow cytometry. The results showed that the expression of GPIb-IX complex could be detected in the lysate and on the surface of CHO cells at 48 hours after transfection. In conclusion, CHO cell models expressing recombinant wild-type or mutation GPIb-IX complex has been successfully constructed.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
Proteínas Recombinantes
/
Cricetulus
/
Células CHO
/
Complejo GPIb-IX de Glicoproteína Plaquetaria
/
Genética
/
Mutación
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2009
Tipo del documento:
Artículo
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