Influence of intra-bone marrow infusion of donor lymphocytes on development of graft-versus-host disease and level of IL-4 and IFN-gamma in peripheral blood / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1538-1540, 2009.
Article
en Zh
| WPRIM
| ID: wpr-328605
Biblioteca responsable:
WPRO
ABSTRACT
To investigate the effect of donor lymphocyte infusion (DLI) by intra-bone marrow (IBM) routes on the incidence of graft-versus-host disease(GVHD), level of IL-4 and IFN-gamma after allogeneic peripheral hematopoietic stem cell transplantation (allo-PBSCT). Female C57BL/6 mice as recipients received total body irradiation (TBI) on day 0, followed by injection of peripheral hematopoietic stem cells from mobilized donor of male BALB/c with the granulocyte-colony stimulating factor (rhG-CSF), and DLI was performed via ether IV or IBM routes. The severity of GVHD was compared in recipients received allogeneic IBM-DLI with those mice received IV-DLI; at 14 days after DLI, the levels of IL-4 and interferon (IFN)-gamma were tested by ELISA. The results showed that as compared with IV-DLI group the frequency and severity of GVHD were reduced in IBM-DLI (p < 0.01); the level of IL-4 significantly increased, while the level of IFN-gamma decreased in group IV-DLI (p < 0.01). It is concluded that IBM-DLI declines the incidence and severity of GVHD after allo-PBSCT.
Texto completo:
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Índice:
WPRIM
Asunto principal:
Fisiología
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Sangre
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Médula Ósea
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Células Madre Hematopoyéticas
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Interleucina-4
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Interferón gamma
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Transfusión de Linfocitos
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Enfermedad Injerto contra Huésped
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Métodos
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Ratones Endogámicos BALB C
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2009
Tipo del documento:
Article