Methylation status of CpG islands in secreted frizzled-related protein gene promoter region of malignant hematopoietic cell lines / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1487-1491, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-328615
ABSTRACT
To investigate the methylation status of CpG islands of the secreted frizzled-related protein (SFRP) gene promoter region in malignant hematopoietic cell lines, and to explore the possible relationship of CpG abnormal methylation status with pathogenic mechanism of hematologic malignancies. Methylation-specific PCR was used to detect the status of SFRP gene promoter region in nine malignant hematopoietic cell lines and peripheral blood mononuclear cells from healthy people. The results indicated that hypermethylation of 2 genes coding for SFRP1 and 2 were present in nine malignant hematopoietic cell lines, however, methylation and unmethylation of SFRP4 were both detected in CA46, HL60 and U937 cell lines, and SFRP5 in U266 as well. None of the normal mononuclear cells showed methylation of SFRP 1-5 genes. It is concluded that the hypermethylation of SFRP genes is related to the evolution of malignant hematopoiesis. Methylation of SFRP genes may serve as potential independent biomarkers for early detection of hematologic malignancies.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Proto-Oncogénicas
/
Regiones Promotoras Genéticas
/
Islas de CpG
/
Neoplasias Hematológicas
/
Metilación de ADN
/
Péptidos y Proteínas de Señalización Intercelular
/
Línea Celular Tumoral
/
Genética
/
Proteínas de la Membrana
Tipo de estudio:
Estudio de tamizaje
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2009
Tipo del documento:
Artículo
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