Comparison of the effect of local administration of endothelial progenitor cells and VEGF on improving neovascularization in flap prefabrication / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 451-455, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-328649
ABSTRACT
<p><b>OBJECTIVE</b>To compare the effect of local administration of endothelial progenitor cells (EPCs) and VECF on improving neovascularization and augmenting the survival areas in a rat model of prefabricated flap.</p><p><b>METHODS</b>Prefabricated flaps were created by ligating the right femoral vascular pedicle and implanting it underneath the abdominal flap. The in vitro cultured EPCs (Group I , n=15) and VEGF protein (Group II , n=15) were injected subcutaneously around the implanted pedicle in experimental groups. PBS was injected in control group (Group Ill , n=15). 4 weeks later, the abdominal island flap based solely on the implanted vessels was elevated and sutured back. Then flap viability and numbers of capillary were evaluated on day 7.</p><p><b>RESULTS</b>There was more statistically significant augmentation of flap survival [(87.26 +/- 10.13) % versus (66.13 +/- 9.9)% and (55.59 +/- 13.06)%, P < 0.001], a higher capillary density (38.67 +/- 9.52 versus 25.83 +/- 6.33 and 26.5 +/- 5.61 capillary/mm2 , P < 0.05) in EPCs group than in the other two groups.</p><p><b>CONCLUSIONS</b>EPCs are superior to VEGF in improving neovascularization during flap prefabrication. Local transplantation of bone marrow-derived EPCs may be a useful strategy for augmentation of the survival areas of prefabricated flaps.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Colgajos Quirúrgicos
/
Endotelio Vascular
/
Células Cultivadas
/
Ratas Wistar
/
Neovascularización Fisiológica
/
Biología Celular
/
Trasplante de Células Madre
/
Factor A de Crecimiento Endotelial Vascular
/
Supervivencia de Injerto
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Plastic Surgery
Año:
2009
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS