Use of specific antibody to detect fetal erythroblasts in maternal circulation / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 494-497, 2004.
Article
en Zh
| WPRIM
| ID: wpr-328840
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the feasibility of using immunohistochemical technique to detect the presence of fetal erythroblasts in the maternal circulation for prenatal diagnosis.</p><p><b>METHODS</b>Maternal blood was obtained from 30 pregnant women at 8 to 26 weeks of gestation. Nucleated red blood cells (NRBCs) were separated with Percoll using a discontinuous density gradient method, and then smeared on microscope slides using cytocentrifugation. Slides were stained with antibody against the gamma-chain of fetal hemoglobin (HbF). All positive NRBCs were collected by micromanipulator under microscopic observation, and then amplified by improved primer extension preamplification(PEP). Sex and Duchenne's musclar dystrophy (DMD) genetic diagnosis were determined from a small aliquot of the PEP reaction.</p><p><b>RESULTS</b>NRBCs stained with HbF were found in all of the blood from the 30 pregnant women at 8 to 26 weeks of gestation. 17 male fetuses and 13 female fetuses were detected in the 30 cases. These results coincided with those of induced labor or amniotic fluid control, and 8 fetuses at the risk of DMD were diagnosed.</p><p><b>CONCLUSION</b>This diagnostic method using immunohistochemical technique to mark fetal NRBC shows good application prospects.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Diagnóstico Prenatal
/
Hemoglobina Fetal
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Inmunohistoquímica
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Eritroblastos
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Edad Gestacional
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Alergia e Inmunología
/
Metabolismo
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Métodos
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Microscopía
/
Anticuerpos Monoclonales
Tipo de estudio:
Diagnostic_studies
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Año:
2004
Tipo del documento:
Article