Effects of Biejiajian Pills on Wnt signal pathway signal molecules β-catenin/TCF4 complex activities and downstream proteins cyclin D1 and MMP-2 in hepatocellular carcinoma cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1758-1762, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-329206
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Biejiajian Pills on Wnt signal pathway and the mechanisms underlying its action to suppress the invasiveness of hepatocellular carcinoma.</p><p><b>METHODS</b>HepG2 cells cultured in the serum of rats fed with Biejiajian Pills for 48 h were examined for β-catenin expression using immunofluorescence, β-catenin/TCF4 complex activity with luciferase, and expressions of the downstream proteins cyclin D1 and MMP-2 using qRT-PCR.</p><p><b>RESULTS</b>Biejiajian Pills-treated sera significantly reduced the expressions of cytoplasmic and nuclear β-catenin protein, cyclin D1 and MMP-2 proteins and lowered the activities of β-catenin/TCF4 complex.</p><p><b>CONCLUSION</b>Biejiajian Pills may serve as a potential anti-tumor agent, whose effect might be mediated by inhibiting the Wnt/β-catenin pathway.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Factores de Transcripción
/
Medicamentos Herbarios Chinos
/
Carcinoma Hepatocelular
/
Ciclina D1
/
Metaloproteinasa 2 de la Matriz
/
Proteínas Wnt
/
Beta Catenina
/
Factores de Transcripción Básicos con Cremalleras de Leucinas y Motivos Hélice-Asa-Hélice
/
Células Hep G2
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2014
Tipo del documento:
Artículo
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