Expression of protease-activated receptor 1 in the basilar artery of rats following subarachnoid hemorrhage / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To assess the relationship between protease-activated receptor 1 (PAR1) expression in the basilar artery and cerebral vasospasm (CVS) in a rat model of subarachnoid hemorrhage (SAH).</p><p><b>METHODS</b>Twenty-four SD rats were randomized into normal control group, SAH 3-days group, SAH 5-days group and SAH 7-days group. Rat models of SAH were established by two injections of blood into the cisterna magna and the behavioral changes of the rats were observed. The basilar arteries were taken at 3, 5, or 7 days following the modeling for measuring the cross-sectional area of the basilar artery and for immunohistochemical detection of PAR1 expression.</p><p><b>RESULTS</b>The SAH model rats, especially those in SAH 3-days group, presented with obvious neurological deficits, which was not found in the normal control group. CVS was not observed in the normal control group but occurred in the SAH model rats, which showed reduced cross-sectional area of the basilar artery and worsening spasm over time. The expression level of PAR1 tended to increase gradually in SAH 3-days, SAH 5-days and SAH 7-days groups. Pearson correlation analysis showed an inverse correlation between the expression of PAR1 and the cross-sectional area of the basilar artery (r=-0.779, P<0.01).</p><p><b>CONCLUSIONS</b>The expression of PAR1 increases significantly in rat basilar artery wall following SAH in positive correlation with the severity of CVS, suggesting the role of thrombin in the pathological process of CVS after SAH.</p>