Correlation of genomic DNA methylation level with unexplained early spontaneous abortion / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1498-1502, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-329260
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation of genomic DNA methylation level with unexplained early spontaneous abortion and analyze the role of DNMT1, DNMT3A and DNMT3B.</p><p><b>METHODS</b>Forty-five villus samples from spontaneous abortion cases (with 33 maternal peripheral blood samples) and 44 villus samples from induced abortion (with 34 maternal peripheral blood samples) were examined with high-pressure liquid chromatography (HPLC) to measure the overall methylation level of the genomic DNA. The expressions of DNMT mRNAs were detected using fluorescence quantitative-PCR in the villus samples from 33 induced abortion cases and 30 spontaneous abortion cases.</p><p><b>RESULTS</b>Genomic DNA methylation level was significantly lower in the villus in spontaneous abortion group than in induced abortion group (P<0.01), but similar in the maternal blood samples between the two groups (P>0.05). The mean mRNA expression levels of DNMT1 and DNMT3A in the villus were significantly lower in spontaneous abortion group than in induced abortion group (P<0.05), but DNMT3B expression showed no significant difference between them (P>0.05).</p><p><b>CONCLUSION</b>Insufficient genomic DNA methylation in the villus does exist in human early spontaneous abortion, and this insufficiency is probably associated with down-regulated expressions of DNMT1 and DNMT3A.</p>
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Aborto Espontáneo
/
Metilación de ADN
/
Genómica
/
ADN (Citosina-5-)-Metiltransferasas
/
ADN (Citosina-5-)-Metiltransferasa 1
/
Genética
/
Metabolismo
Límite:
Femenino
/
Humanos
/
Embarazo
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2014
Tipo del documento:
Artículo
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