Study on exons 3 and 4 of alpha-synuclein gene in Chinese familial Parkinson disease patients / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 536-538, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-329417
ABSTRACT
<p><b>OBJECTIVE</b>To study the relationship of Chinese familial Parkinson disease with alpha-synuclein gene.</p><p><b>METHODS</b>Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex analysis(PCR-HA) were employed to detect the abnormal mobilization in the familial Parkinson disease and sporadic Parkinson disease patients, then it was verified by gene sequencing.</p><p><b>RESULTS</b>No mutation was found in alpha-synuclein gene exons 3 and 4 by PCR-SSCP together with PCR-HA. An inserted c and an inserted t were found in intron 4, position 23 and position 67 respectively.</p><p><b>CONCLUSION</b>(1) Exons 3 and 4 of alpha-synuclein gene are not the mutational hot spots of Chinese familial Parkinson disease. (2) Two polymorphisms were found in intron 4 of alpha-synuclein gene. They are 23 ins c and 67 ins t.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Enfermedad de Parkinson
/
Reacción en Cadena de la Polimerasa
/
Exones
/
Polimorfismo Conformacional Retorcido-Simple
/
Análisis Heterodúplex
/
Sinucleínas
/
Alfa-Sinucleína
/
Genética
/
Mutación
/
Proteínas del Tejido Nervioso
Límite:
Adulto
/
Anciano
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2003
Tipo del documento:
Artículo
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