Detecting MTHFR gene mutation with a new approach: molecular beacons / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 436-437, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-329441
ABSTRACT
<p><b>OBJECTIVE</b>To detect the methylenetetrahydrofolate reductase(MTHFR) gene C677T mutation with molecular beacon technique and assess the revant applicability.</p><p><b>METHODS</b>A total of 228 samples were analyzed using molecular beacons which are oligonucleotide probes to become fluorescent upon hybridization. Wild-type molecular beacon and mutant beacon were designed to detect the genotypes of MTHFR gene.</p><p><b>RESULTS</b>Analysis of the 228 samples indicated that there were three genotypes including 41 homozygous mutants, 113 heterozygous individuals and 74 wild-type individuals. Every sample was identified clearly.</p><p><b>CONCLUSION</b>The present method, a closed-tube PCR/hybridization assay, is a simple, high-throughput and fast procedure that is fully automated for detecting gene mutation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Análisis Mutacional de ADN
/
Sondas de Oligonucleótidos
/
Química
/
Sensibilidad y Especificidad
/
Mutación Puntual
/
Metilenotetrahidrofolato Reductasa (NADPH2)
/
Colorantes Fluorescentes
/
Genética
/
Genotipo
/
Métodos
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2003
Tipo del documento:
Artículo
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