Establishment and preliminary application of screening methods for Y chromosome microdeletions in male infertility patients / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 357-359, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-329461
ABSTRACT
<p><b>OBJECTIVE</b>To develop a multiplex PCR protocol, which could be suitable for routine screening of microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.</p><p><b>METHODS</b>Five multiplex sets were established. Eighty-seven azoospermic and oligozoospermic patients undergoing intracytoplasmic sperm injection (ICSI) in the in vitro fertilization (IVF) center and 30 azoospermic men undergoing testicular biopsy in the clinic of Urology Surgery were screened for microdeletions of Y chromosome.</p><p><b>RESULTS</b>A total of 19 (16.2%) cases of microdeletions were found in 117 azoospermic and oligozoospermic patients by screening of Y chromosome microdeletions. Of these, 11 cases (18.0%) were found in 61 oligozoospermic patients, and 8 cases (14.3%) were found in 56 azoospermic patients.</p><p><b>CONCLUSION</b>The multiplex PCR protocol presented in this study is an easy-to-do and reliable method for detecting microdeletions on the Y chromosome. Routine screening of microdeletions on the Y chromosome for azoospermic and oligozoospermic patients is essential.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Pruebas Genéticas
/
Reacción en Cadena de la Polimerasa
/
Deleción Cromosómica
/
Cromosomas Humanos Y
/
Diagnóstico
/
Genética
/
Infertilidad Masculina
/
Métodos
Tipo de estudio:
Estudio diagnóstico
/
Guía de Práctica Clínica
/
Estudio de tamizaje
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Medical Genetics
Año:
2003
Tipo del documento:
Artículo
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