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Serial analysis of gene expression in Monascus aurantiacus producing citrinin / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 9-14, 2005.
Artículo en Inglés | WPRIM | ID: wpr-329607
ABSTRACT
<p><b>OBJECTIVE</b>To construct a tag expression library of Monascus aurantiacus that could produce citrinin maximally on the thirteenth (0.966 mg/mL) day in the submerged culture.</p><p><b>METHODS</b>Total RNA was extracted from the mycelium, cDNA was synthesized using the SuperScript choice system, and then, a SAGE library was successfully constructed according to the MicroSAGE method.</p><p><b>RESULTS</b>Five hundred and ninety eight clones were obtained in SAGE library, and 120 clones were picked out randomly for identification and sequencing purpose. Eighty nine clones had positive inserts, 26 clones had no inserts and the remaining 5 clones had no site of NlaIII enzyme in inserts. There were seven repeated clones.</p><p><b>CONCLUSION</b>With the aid of SAGE2000 software, 901 tags were obtained from 89 clones, representing 686 unique transcripts. Six unique tags of them belong to highly expressed genes (Number of tags > or = 10) and 143 unique tags to moderately expressed genes (repeat tags > or = 2).</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ARN de Hongos / Expresión Génica / Biblioteca de Genes / Reacción en Cadena de la Polimerasa / Citrinina / Etiquetas de Secuencia Expresada / Perfilación de la Expresión Génica / Monascus / Genética / Metabolismo Idioma: Inglés Revista: Biomedical and Environmental Sciences Año: 2005 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: ARN de Hongos / Expresión Génica / Biblioteca de Genes / Reacción en Cadena de la Polimerasa / Citrinina / Etiquetas de Secuencia Expresada / Perfilación de la Expresión Génica / Monascus / Genética / Metabolismo Idioma: Inglés Revista: Biomedical and Environmental Sciences Año: 2005 Tipo del documento: Artículo