Effects of aging and advanced glycation on gene expression in cerebrum and spleen of mice / 生物医学与环境科学（英文）

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the effects of aging or advanced glycation on gene expression in the cerebrum and spleen of female C57BL/6J mice.</p><p><b>METHODS</b>The gene expression profile was determined by using cDNA expression arrays containing 588 cDNA.</p><p><b>RESULTS</b>Aging and advanced glycation resulted in differential gene expression patterns of cerebrum and spleen compared with young mice. Among the 80 genes detected in cerebrum, 43 exhibited a change in mRNA ratios with aging or treatment. Thirty-four changes (79%) were common in aged and D-galactose treated mice, whereas the cerebrum from aged and AGE-lysine treated mice showed common changes in expression of 38 genes (88%). Of the 86 genes detected in spleen, 29 (34%) displayed an age-related decrease in expression, whereas 3 (3%) displayed an increase in expression levels with aging. Eighteen genes from the detectable genes exhibited expression changes in both cerebrum and spleen of mice.</p><p><b>CONCLUSIONS</b>The gene expression profiles of D-galactose and AGE-lysine treated mice resemble those of aged mice. Use of cDNA hybridization arrays may provide a promising tool to explore the mechanism of aging at a molecular level.</p>