Effects of CGRP on the E-cadherin expression in human bronchial epithelial cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 346-349, 2012.
Article
en Zh
| WPRIM
| ID: wpr-329867
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To discuss the effect of calcitonin gene-related peptides (CGRP) on epithelial cadherin (E-cd) expression in human bronchial epithelial cells (HBECs) in vitro.</p><p><b>METHODS</b>The effect of CGRP on E-cd protein and mRNA expression in both normal and O3-challenged HBECs were determined by immunocytochemistry and RT-PCR. The signal transduction pathways of CGRP were observed by using protein kinase C(PKC) inhibitor (H-7), calmodulin(CaM) inhibitor (W-7) and PKA inhibitor (H-89).</p><p><b>RESULTS</b>CGRP increased E-cd mRNA and protein expressions of normal and O3-challenged HBECs in a dose-dependent manner. CGRP had no effect on cytoplasm E-cd expression. Pre-treatment with H-89, H-7 and W-7, the up-regulatory effect of CGRP on E-cd expression was partly abolished.</p><p><b>CONCLUSION</b>CGRP increased in cytomembrane E-cd expression of normal and O3-challenged HBECs in a dose-dependent manner. E-cd expression on HBECs was strengthened by CGRP via PKA, PKC and CaM pathways.</p>
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Índice:
WPRIM
Asunto principal:
Ozono
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Farmacología
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Bronquios
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ARN Mensajero
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Cadherinas
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Péptido Relacionado con Gen de Calcitonina
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Línea Celular
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Biología Celular
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Células Epiteliales
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Genética
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2012
Tipo del documento:
Article