The attenuation of myocardial susceptibility to ischemia/reperfusion injury by ischemic postconditioning in hypercholesteremia rats and the role of hypoxia inducible factor-1alpha / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 319-327, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-329875
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether ischemic postconditioning can attenuate the myocardial injury induced by ischemia/reperfusion (I/R) in hypercholesteremic rats and whether hypoxia inducible factor-1alpha (HIF-1alpha) play a role in the protection.</p><p><b>METHODS</b>Adult male Wistar rats received a high fat diet for 8 weeks to prepare the hypercholesteremic models. Myocardial damage induced by ischemia/reperfusion was evaluated by infarct size, creatine kinase (CK) activity and myocardial apoptosis. HIF-1alpha mRNA level was detected by real time-RT-PCR and the protein level was detected by Western blot.</p><p><b>RESULTS</b>Myocardial infarct size, CK activity, and caspase-3 activity induced by I/R were markedly increased in hypercholesteremic rats compared with those in normal rats. Ischemic postconditioning attenuated the myocardial injury in both normal rats and hypercholesteremic rats, and increased HIF-1alpha protein level. There was a significant linear inverse relationship between HIF-1alpha protein level and infarct size (r = -0.802, P <0.01).</p><p><b>CONCLUSION</b>Hypercholesteremia enhanced the susceptibility of myocardia to ischemia/reperfusion injury. While ischemic postconditioning markedly attenuated the increase of myocardial susceptibility to I/R induced by hypercholesteremia. HIF-1alpha might be one of the mechanisms of protection by ischemic postconditioning.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Daño por Reperfusión Miocárdica
/
Ratas Wistar
/
Apoptosis
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Creatina Quinasa
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Susceptibilidad a Enfermedades
/
Subunidad alfa del Factor 1 Inducible por Hipoxia
/
Caspasa 3
/
Poscondicionamiento Isquémico
/
Hipercolesterolemia
/
Metabolismo
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Applied Physiology
Año:
2012
Tipo del documento:
Artículo
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