A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 510-512, 2011.
Article
en Zh
| WPRIM
| ID: wpr-329963
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.</p><p><b>METHODS</b>DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.</p><p><b>RESULTS</b>The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.</p><p><b>CONCLUSION</b>The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Fisiología
/
Activación del Canal Iónico
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Separación Celular
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Ratas Sprague-Dawley
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Técnicas de Placa-Clamp
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Biología Celular
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Ganglios Espinales
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Canales Iónicos
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Métodos
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Neuronas
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2011
Tipo del documento:
Article