Cloning and sequence analysis of recombinant fusion gene of Escherichia coli heat-liable enterotoxin B subunit and Actinobacillus actinomycetemcomitans fimbria associative protein / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 24-40, 2005.
Article
en Zh
| WPRIM
| ID: wpr-329997
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clone the recombinant fusion gene of Escherichia coli heat-liable enterotoxin B subunit (Ltb) and Actinobacillus actinomycetemcomitans fimbria associative protein (Fap).</p><p><b>METHODS</b>Two couples of primers were designed for PCR according to the known sequence of ltb and fap. The ltb and fap gene were obtained by amplification PCR technique from plasmid EWD299 of Escherichia coli and Actinobacillus actinomycetemcomitans 310 DNA respectively, and fused them by PCR. The fusion gene ltb-fap were cloning into plasmid pET28a(+). The recombined plasmid pET28a ltb-fap was transformed into Escherichia coli DH5alpha. The recombinant was screened and identified by restriction enzyme and PCR. The cloned gene was sequenced.</p><p><b>RESULTS</b>The ltb-fap about 531bp in size was obtained successfully, and identified by PCR, restrictive enzyme and sequence analysis.</p><p><b>CONCLUSION</b>The vector of pET28a ltb-fap was obtained.</p>
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Plásmidos
/
Toxinas Bacterianas
/
Proteínas Recombinantes de Fusión
/
Reacción en Cadena de la Polimerasa
/
Clonación Molecular
/
Aggregatibacter actinomycetemcomitans
/
Análisis de Secuencia
/
Clonación de Organismos
/
Proteínas de Escherichia coli
/
Enterotoxinas
Idioma:
Zh
Revista:
West China Journal of Stomatology
Año:
2005
Tipo del documento:
Article