Synthesis and identification of artificial antigen of forsythin / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2295-2299, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-330303
ABSTRACT
The establishment of high specificity and sensitivity method of small molecule monoclonal antibody-based immunoassay has a great importance in the study of small molecule compounds in Chinese medicine, wherein synthesis of small molecule artificial antigen is a critical step in the preparation of small molecule antibodies. Oxidation method using sodium iodide was used to synthesize immunogenic antigen (FRn-BSA) and coating antigen (FRn-OVA) of forsythin. UV spectroscopy and thin layer chromatography showed that forsythin was successfully conjugated with BSA and OVA. After immuned FRn-BSA, the mice could specifically produce anti-forsythin antibodies with titer up to 18 000, and the linear range was from 1 mg x L(-1) to 100 mg x L(-1). In this paper, the artificial antigen of forsythin was successfully synthesized, which can be applied for preparation of monoclonal antibodies and establishment of appropriate immune method.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Medicamentos Herbarios Chinos
/
Química
/
Compuestos Bicíclicos Heterocíclicos con Puentes
/
Alergia e Inmunología
/
Furanos
/
Ratones Endogámicos BALB C
/
Anticuerpos
/
Antígenos
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2014
Tipo del documento:
Artículo
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