Identification of Bupleurum chinense and B. longiradiatum based on ITS2 barcode / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2160-2163, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-330329
ABSTRACT
In this study, ITS2 barcode was used to identify Bupleurum chinense and B. longiradiatum. The ITS2 regions of 48 samples were amplified and sequenced. The sequences obtained above were aligned and the K2P distances were calculated. We used three methods, BLAST1, nearest distance and phylogenetic tree (NJ-tree), to test the identification ability. The results showed that the maximum intraspecific genetic distance of B. chinense was 0.013, and the minimum interspecific genetic distance between B. chinense and B. longiradiatum was 0.049. The NJ-tree can easily identify B. chinense and B. longiradiatum. Therefore, the ITS2 barcode is suitable to identify B. chinense and B. longiradiatum.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Control de Calidad
/
Medicamentos Herbarios Chinos
/
Datos de Secuencia Molecular
/
Química
/
Clasificación
/
ADN de Plantas
/
ADN Espaciador Ribosómico
/
Bupleurum
/
Código de Barras del ADN Taxonómico
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2014
Tipo del documento:
Artículo
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