Screening bioactive compounds inhibiting influenza virus from isatidis radix by ultrafiltration mass spectrometry / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 812-816, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-330356
ABSTRACT
In vitro neuraminidase inhibition assays and ultrafiltration liquid chromatography with diodearray detector coupled to time of flight mass spectrometer (UPLC-DAD-TOF-MS) were combined to screen bioactive compounds inhibiting neuraminidase from Isatidis Radix. By comparing the compounds from Isatidis Radix before and after ultrafiltration, we found that arginine, goitrin and adenosinea can bind with neuraminidase, and the binding degree of the three compounds were (36.23 +/- 1.12)%, (32.54 +/- 1.02)% and (9.38 +/- 0.47)%, respectively. The IC50 of arginine and goitrin were (1.16 +/- 0.02), (1.20 +/- 0.02) g x L(-1), respectively. While the IC50 of adenosinea was higher than 500 g x L(-1). The results showed that arginine and goitrin might be the main compounds with antiviral activity of Isatidis Radix. This study may provide a useful method for the screening of bioactive compounds and quality control of Isatidis Radix.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Antivirales
/
Orthomyxoviridae
/
Farmacología
/
Arginina
/
Espectrometría de Masas
/
Proteínas Virales
/
Medicamentos Herbarios Chinos
/
Ultrafiltración
/
Química
/
Raíces de Plantas
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2014
Tipo del documento:
Artículo
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