Norcantharidin inhibits DNA replication initiation protein Cdc6 in cancer cells / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To explore the inhibitory effect of norcantharidin (NCTD) on the expression of DNA replication initiation protein Cdc6 in cancer cells.</p><p><b>METHODS</b>MTT assay was performed to detect the inhibitory effect on different cancer cell lines, including HeLa, HepG2, Jurkat and Ramos cells. The effect of NCTD on Cdc6 protein level was detected by Western blotting, and BrdU incorporation assay was used to evaluate the DNA replication of the cells.</p><p><b>RESULTS</b>NCTD significantly inhibited the proliferation of the cells and caused degradation of Cdc6 protein to result in the inhibition of the DNA replication of the cells shown by BrdU incorporation assay.</p><p><b>CONCLUSION</b>NCTD can induce the degradation of Cdc6 in cancer cells to produce an anti-cancer effect.</p>