Down-regulation of CD147 expression induces SiHa cell apoptosis through the Bcl-2 pathway / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1695-1698, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-330855
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in SiHa cell apoptosis after inhibition of CD147 expression.</p><p><b>METHODS</b>RNA interference (RNAi) technique was used to down-regulate CD147 expression in SiHa cells, and RT-PCR and Western blotting were used to detect expression of CD147, Bcl-2, Bim and caspase-3; the percentage of cell apoptosis were detected by flow cytometry.</p><p><b>RESULTS</b>SiRNA sequence 1, 2 inhibited CD147 expression in SiHa cells effectively (P<0.05), resulting also in down-regulated expression of Bcl-2 (P<0.05) and up-regulated expression of caspase-3 and Bim(P<0.05). The percentage of apoptotic cells increased significantly, and early apoptosis was the most obvious in the cells (P<0.05).</p><p><b>CONCLUSION</b>Silencing of CD147 expression induces SiHa cell apoptosis partially through the Bcl-2 pathway .</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Regulación hacia Abajo
/
Proteínas Proto-Oncogénicas
/
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
/
Interferencia de ARN
/
Línea Celular Tumoral
/
Proteínas Reguladoras de la Apoptosis
/
Basigina
/
Caspasa 3
/
Proteína 11 Similar a Bcl2
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2010
Tipo del documento:
Artículo
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