Effect of lentinan against immunosuppression of lymphocytes cultured in simulated microgravity environment / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 182-186, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-330994
ABSTRACT
This study was aimed to evaluate the effect of lentinan on the immune function of splenic lymphocytes in rotary cell culture system (RCCS) microgravity environment. The splenic lymphocytes from mice were separated and cultured in the normal gravity and the microgravity environments. The cells were treated with lentinan solution (0, 10, 20 and 40 µg/ml). After incubated with lentinan for indicated times (24, 48 and 72 h), the cell proliferation, secretion of cytokine and the expression of cell surface markers were detected by MTT method, ELISA and flow cytometry respectively. The results indicated that lentinan of above mentioned concentrations did not obviously promote the lymphocyte proliferation, but increased the secretion of IL-2 and IFN-γ and enhanced the expression of lymphocyte surface markers CD4 and CD8 in microgravity environment. It is concluded that lentinan has the ability to enhance the lymphocyte immune function in microgravity environment.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Bazo
/
Activación de Linfocitos
/
Linfocitos
/
Células Cultivadas
/
Citocinas
/
Terapia de Inmunosupresión
/
Simulación de Ingravidez
/
Secreciones Corporales
/
Biología Celular
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2012
Tipo del documento:
Artículo
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