Discrimination of alleles in HLA-C*07:01:01G and HLA-C*07:02:01G groups through detection sequences in exons 1 to 7 of HLA-C locus by using polymerase chain reaction sequence-based typing / 中国实验血液学杂志

ABSTRACT

This study was aimed to discriminate the alleles in the HLA-C*070101G and HLA-C*070201G groups and analyze their associations with HLA-B locus. Samples previously typed as HLA-C*070101G and HLA-C*070201G were collected. The nucleotide sequences in exons 1 to 7 of the HLA-C locus were sequenced by polymerase chain reaction sequence-based typing (PCR-SBT) and HLA-B genotyping was also preformed by PCR-SBT in these samples. The results showed that 4 samples (30.8%) were confirmed as HLA-C*070101 and 9 samples (69.2%) were HLA-C*0706 among 13 samples previously typed as HLA-C*070101G. Linkage disequilibrium (LD) analysis showed that HLA-C*0706 allele was strongly related with HLA-B*4403. All samples were typed as C*070201 among 102 individuals previously typed as C*070201G. LD analysis showed that C*070201 was strongly related with HLA-B*5101, B*4601, B*3901, B*4001, B*3802, B*1502 alleles. It is concluded that HLA-C*070101 and HLA-C*0706 alleles are confirmed in the HLA-C*070101G group and HLA-C*070201 is a preferred allele in the HLA-C*070201G.