RQ-PCR detection of GST-π and LRP genes in adult acute leukemia and its clinical significance / 中国实验血液学杂志

ABSTRACT

This study was aimed to detect the glutathione S-transferase-π (GST-π) and lung resistance-related protein (LRP) genes and to investigate their relationship with multidrug resistance (MDR) of patients with acute leukemia (AL). Real-time fluorescent quantitative reverse transcription polymerase chain reaction (RQ-PCR) was used to detect the expression of GST-π and LRP genes in peripheral blood mononuclear cells from 44 AL patients and 27 normal subjects. The results showed that the significant difference in GST-π expression level was found between newly diagnosed patients and complete remission patients and between refractory patients and complete remission patients (P < 0.01), while expression level of LRP genes showed obvious difference (P ≤ 0.01) between newly diagnosed patients and refractory patients and between complete remission patients and refractory patients. Statistical analysis indicated that there was no correlation between GST-π gene and LRP gene. The expression of GST-π and LRP genes was not significantly different in different white blood cell (WBC) count groups and different clinical typing groups (ALL and ANLL). It is concluded that the mechanism of MDR resulting from GST-π and LRP genes is different, thereby combination detection of GST-π and LRP genes demonstrates a larger role for evaluating prognosis of AL patients, as compared with detection of GST-π or LRP gene alone. The WBC count and leukemia typing have no relationship with expression of GST-π and LRP genes.