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Influence of mesenchymal stem cells on proliferation of HL-60 / 中国实验血液学杂志
Article en Zh | WPRIM | ID: wpr-331021
Biblioteca responsable: WPRO
ABSTRACT
The objective of this study was to explore the effect of mesenchymal stem cells (MSC) on HL-60 proliferation and its molecular mechanism. HL-60 cells co-cultivated with MSC were used as experiment group, and the cells cultivated solely were taken as control group. HL-60 cells in the two groups were counted at different time. The time-quantity curve was drawn. The cell cycle and apoptosis ratio of HL-60 cells were compared between the two groups and expressions of Survivin and Bcl-2 protein were detected by flow cytometry. The results showed that HL-60 cells cultivated with MSC were obviously inhibited, especially on day 5 and 7 (P < 0.01). HL-60 cells were distributed on the phase of G(0)/G(1) [control group (47.0 ± 9.0)% vs experiment group (70.0 ± 16.0)%, P = 0.003], and apoptotic peak appeared. Both of Survivin and Bcl-2 protein expressions in HL-60 cells decreased [Bcl-2 protein in control group (63.0 ± 9.1)% vs experiment group (50.0 ± 14.1)%, P = 0.045; Survivin in control group (94.0 ± 9.3)% vs experiment group (77.0 ± 11.8)%, P = 0.006]. It is concluded that the MSC can inhibit HL-60 cell proliferation, and promote HL-60 cell apoptosis.
Asunto(s)
Texto completo: 1 Índice: WPRIM Asunto principal: Células de la Médula Ósea / Apoptosis / Células HL-60 / Técnicas de Cocultivo / Proteínas Proto-Oncogénicas c-bcl-2 / Biología Celular / Proliferación Celular / Proteínas Inhibidoras de la Apoptosis / Células Madre Mesenquimatosas / Citometría de Flujo Límite: Adult / Humans Idioma: Zh Revista: Journal of Experimental Hematology Año: 2012 Tipo del documento: Article
Texto completo: 1 Índice: WPRIM Asunto principal: Células de la Médula Ósea / Apoptosis / Células HL-60 / Técnicas de Cocultivo / Proteínas Proto-Oncogénicas c-bcl-2 / Biología Celular / Proliferación Celular / Proteínas Inhibidoras de la Apoptosis / Células Madre Mesenquimatosas / Citometría de Flujo Límite: Adult / Humans Idioma: Zh Revista: Journal of Experimental Hematology Año: 2012 Tipo del documento: Article