Application of reverse transcription-multiplex nested PCR to detect PDGFRB gene rearrangement in myeloproliferative disorders / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1443-1446, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-331058
ABSTRACT
In order to explore the value of reverse transcription(RT)-multiplex nested PCR for detecting PDGFRB gene rearrangement in myeloproliferative disorders (MPD), the PDGFRB rearrangement was detected qualitatively in 146 MPD cases by reverse transcription multiplex nested PCR. The results showed that 8 cases with PDGFRB fusion gene were found in 146 cases, the positive rate was 5.5%. Out of 8 cases with PDGFRB fusion gene, TEL-PDGRB fusion gene was found in 3 cases; HIP1-PDGFRB fusion gene in 2 cases; GIT2-PDGFRB, TP53BP1-PDGFRB and WDP48-PDGFRB fusion gene in 1 case, respectively. It is concluded that RT-multiplex nested PCR is a powerful tool for the detection of PDGFRB rearrangement, which helps to tentatively diagnose MPD and to provide the clues for targeting therapy.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Reordenamiento Génico
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Receptor beta de Factor de Crecimiento Derivado de Plaquetas
/
Diagnóstico
/
Reacción en Cadena de la Polimerasa Multiplex
/
Genética
/
Métodos
/
Trastornos Mieloproliferativos
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2011
Tipo del documento:
Artículo
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