Changes in telomerase activity decreases in MCF-7 cells during apoptosis induced by sodium butyrate / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 9-12, 2005.
Article
en Zh
| WPRIM
| ID: wpr-331254
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate telomerase activity of MCF-7 mammary cancer cells during apoptosis induced by sodium butyrate (SB) in vitro and its mechanism.</p><p><b>METHODS</b>The proliferative activity of MCF-7 cells was assessed by morphology and MTT assay. Cell apoptosis was confirmed by DNA fragmentation and phosphatidylserine (PS) externalization. Telomerase activity was examined by TRAP-ELISA. The expression status of telomerase subunits was analyzed by RT-PCR.</p><p><b>RESULTS</b>A time- and dose-dependent inhibition was detected in MCF-7 cells treated with SB. At 72 hr after SB (2.5 mmol/L) treatment, MCF-7 cells were apoptotic with a rate of 84.3% by flow cytometric assay (AnnexinV/PI double staining). Apoptosis was also confirmed by DNA fragmentation. Telomerase activity and expression level of hTERT, the key subunit of telomerase, decreased at 24-hour time point after SB treatment. No significant changes were observed in the expression of hTR and hTP, the other two subunits of telomerase.</p><p><b>CONCLUSION</b>Telomerase activity decreases in MCF-7 cells during apoptosis induced by sodium butyrate. The underlying mechanism might be related to the down regulation of hTERT transcription.</p>
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Índice:
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Asunto principal:
Patología
/
Farmacología
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Factores de Tiempo
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Neoplasias de la Mama
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Butiratos
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ARN Mensajero
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Apoptosis
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Telomerasa
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Línea Celular Tumoral
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Proteínas de Unión al ADN
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Año:
2005
Tipo del documento:
Article