Methods of isolation, expansion, differentiating induction and preservation of human umbilical cord mesenchymal stem cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 4504-4510, 2012.
Artículo
en Inglés
| WPRIM
| ID: wpr-331346
ABSTRACT
<p><b>OBJECTIVE</b>This literature review aims to summarize the methods of isolation, expansion, differentiation and preservation of human umbilical cord mesenchymal stem cells (hUCMSCs), for comprehensive understanding and practical use in preclinical research and clinical trials.</p><p><b>DATA SOURCES</b>All the literature reviewed was published over the last 10 years and is listed in PubMed and Chinese National Knowledge Infrastructure (CNKI). Studies were retrieved using the key word "human umbilical cord mesenchymal stem cells".</p><p><b>RESULTS</b>Explants culture and enzymatic digestion are two methods to isolate hUCMSCs from WJ and there are modifications to improve these methods. Culture conditions may affect the expansion and differentiating orientations of hUCMSCs. In addition, hUCMSCs can maintain their multi-potential effects after being properly frozen and thawed.</p><p><b>CONCLUSION</b>Considering their multi-potential, convenient and non-invasive accessibility, low immunogenicity and the reported therapeutic effects in several different preclinical animal models, hUCMSCs have immense scope in regeneration medicine as a substitute for MSCs derived from bone marrow or umbilical cord blood.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Cordón Umbilical
/
Diferenciación Celular
/
Técnicas de Cultivo de Célula
/
Biología Celular
/
Células Madre Mesenquimatosas
/
Métodos
Límite:
Humanos
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2012
Tipo del documento:
Artículo
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