The effect of microencapsulated NGF-expressing NIH-3T3 cells on bioengineered dermis function in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 61-65, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-331394
ABSTRACT
Nerve growth factor (NGF) can promote the regeneration of peripheral nerve as well as contraction and reepithelization of wound. We constructed a bioengineered dermis containing microencapsulated NGF-expressing NIH-3T3 cells and study the effect of the microencapsule to the bioengineered dermis and seed cells. NGF gene was transfected into NIH-3T3 cells and enclosed into alginate-poly-L-lysine-alginate (APA) microencapsulation and cultivated in vitro. Content of NGF in microencapsules culturing supernatant was measured by enzyme linked immunosorbent assay (ELISA) method. These microencapsules were co-cultured with epidermic cells and fibroblast cells. Bioengineered dermis was constructed with NGF-expressing micorencapsules as seed cells using tissue engineering method. NIH-3T3 microencapsules, empty microencapsules, normal culture media were control groups. After one week culture, the characteristics of the dermis were described by MTT test, the content of hydroxyproline (HP), HE staining and ultrastructure photograph. We found the NGF-expressing microencapsulates can secret NGF steadly after cultured 8w in vitro, promot the proliferation of epidermic cells and secret collagen of fibroblast cells. These functions can maintaine in bioengineered dermis. So NGF-expressing NIH-3T3 microencapsulates can promote the quality of bioengineered dermis.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Polilisina
/
Fenómenos Fisiológicos de la Piel
/
Materiales Biocompatibles
/
Transfección
/
Química
/
Regulación de la Expresión Génica
/
Dermis
/
Factor de Crecimiento Nervioso
/
Biología Celular
/
Ingeniería de Tejidos
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2007
Tipo del documento:
Artículo
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