Influence of human C-type natriuretic peptide on vascular endothelial cell proliferation / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 191-194, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-331499
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of human C-type natriuretic peptide (hCNP) on proliferation of vascular endothelial cells (HUVECs).</p><p><b>METHODS</b>Reconstructed pcDNA3.1 (+)/hCNP was transfected into HUVECs with polyethylenimine and its plasmid expression was examined with RT-PCR, immunohistochemistry and Western blot. MTT method was used to determine the effect of expressed protein on proliferation of HUVECs. pcDNA3.1 (+)/hCNP transfection was used for control.</p><p><b>RESULTS</b>The proliferation of HUVEC 48 h after pcDNA3.1 (+)/hCNP transfection was (0.301 +/- 0.096), which was obviously higher than that with pcDNA3.1 (+) transfection (0.164 +/- 0.012). Reconstructed pcDNA3.1 (+)/hCNP might be expressed in HUVECs effectively and its protein expression was capable of promoting HUVECs proliferation markedly.</p><p><b>CONCLUSION</b>The successive expression of reconstructed pcDNA3.1 (+)/hCNP and the promoting activity of its expressed protein on HUVECs lay the foundation potential therapeutic value of C-type natriuretic peptide.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plásmidos
/
ARN Mensajero
/
Transfección
/
Línea Celular
/
Péptido Natriurético Tipo-C
/
Biología Celular
/
Células Endoteliales
/
Proliferación Celular
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Burns
Año:
2007
Tipo del documento:
Artículo
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