Effects of Panax notoginseng on the transdifferentiation of fibroblasts in human hypertrophic scar in vitro / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 188-190, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-331500
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of Panax notoginseng on the transdifferentiation of the cultured human fibroblasts from hypertrophic scar in vitro, and explore its anti-fibrosis mechanism.</p><p><b>METHODS</b>The fibroblasts from human hypertrophic scar were cultured in vitro. Different amount of Panax notoginseng was added into the medium, respectively (400 microg/ml and 800 microg/ml). A culture without addition of the drug served as control. The fibroblast-populated collagen lattice method was used to detect the gel contraction, and contraction ratio was calculated. The immunocytochemistry staining method was used to detect the expression of alpha-smooth muscle actin. The flow cytometry method was used to detect the positive rate of alpha-smooth muscle actin.</p><p><b>RESULTS</b>The contraction degree of the fibroblasts after PNS administration was ameliorated at each time-point, with contraction index lower than that of controls (P < 0.05 or P < 0.01). Scattered distribution of alpha-SMA positive granules were observed in the cytoplasma, and the positive rate of alpha-SMA expression in 400 microg/ml (31.52%) and 800 microg/ml (24.28%) PNS groups were obviously lower than that in control group (45.74%, P < 0.05). The staining intensity of positive cells in 400 microg/ml and 800 microg/ml PNS groups was also obviously lower than that in control group (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>Panax notoginseng can inhibit the transdifferentiation of the cultured human fibroblasts from hypertrophic scar, and it exhibits an anti-fibrosis effect on hypertrophic scar in vitro.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Cicatrización de Heridas
/
División Celular
/
Células Cultivadas
/
Química
/
Cicatriz Hipertrófica
/
Biología Celular
/
Ginsenósidos
/
Panax notoginseng
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Burns
Año:
2007
Tipo del documento:
Artículo
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