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Expression of tumor necrosis factor related apoptosis inducing ligand and its receptor in thymus during early post-burn stage in rat with severe burns / 中华烧伤杂志
Chinese Journal of Burns ; (6): 184-187, 2007.
Artículo en Chino | WPRIM | ID: wpr-331501
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of tumor necrosis factor related apoptosis inducing ligand (TRAIL) and its receptor on apoptosis in thymus during early post-burn stage in rat with severe burns.</p><p><b>METHODS</b>Fifty Wistar rats were randomly divided into sham scald group (SS, n = 10) and burn group (n = 40). The apoptosis in thymus in rats was detected with annexin V/FITC-PI double staining at 4, 12, 24, 48 post-burn hours (PBH). The expression of TRAIL death receptor DR5, DR4 and its decoy receptor DcR1, DcR2 in thymus were detected by RT-PCR and Western blot at above time-points.</p><p><b>RESULTS</b>Compared with that in SS group (6.7 +/- 0.8)%, the apoptosis in the thymus in burn group started to increase at 4 PBH [(17.1 +/- 0.4)%], peaked at 12 PBH [(25.2 +/- 1.1)%], and it was still evidently higher than that in SS group at 48 PBH (P < 0.05). There was no obvious difference in the apoptosis rate in rats in burn group among all the time-points. The expression of DR5 in burn group at each time-points was significantly higher than those in SS group, while that of DcR2 shown an opposite tendency (P < 0.05). The expression of DR4, DcR1 was similar in both groups.</p><p><b>CONCLUSION</b>The marked increase in apoptosis rate in rat thymus at early post-burn stage, and the significant change in the expression of DR5 and DcR2 show that TRAIL pathway may participate in apoptosis.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Timo / Quemaduras / Ratas Wistar / Apoptosis / Modelos Animales de Enfermedad / Ligando Inductor de Apoptosis Relacionado con TNF / Receptores del Ligando Inductor de Apoptosis Relacionado con TNF / Genética / Metabolismo Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Burns Año: 2007 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Timo / Quemaduras / Ratas Wistar / Apoptosis / Modelos Animales de Enfermedad / Ligando Inductor de Apoptosis Relacionado con TNF / Receptores del Ligando Inductor de Apoptosis Relacionado con TNF / Genética / Metabolismo Tipo de estudio: Estudio pronóstico Límite: Animales Idioma: Chino Revista: Chinese Journal of Burns Año: 2007 Tipo del documento: Artículo