Effect of sodium valproate on human myelodysplastic syndrome cell line SKM-1 and its mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1515-1519, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-332327
ABSTRACT
This study was aimed to investigate the effect of sodium valproate(VPA) on human myelodysplastic syndrome cell line SKM-1 and its mechanism. The cell proliferation was determined by MTT assay, cell apoptosis was analyzed by flow cytometry. The expressions of c-flipl, c-flips and dlk1 mRNA were detected by RT-PCR. The results showed that VPA could inhibited the growth of SKM-1 cells in dose- and time-dependent manners. The flow cytometric analysis indicated that VPA could induce cell apoptosis, apoptosis rate increased in dose-dependent manner. The expressions of c-flipl, c-flips and dlk1 mRNA in SKM-1 cell treated with VPA decreased using of VPA. It is concluded that VPA can induce apoptosis and inhibited proliferation of SKM-1 cells. In this process, the decreasing of c-flipl, c-flips and dlk1 mRNA expression may play important roles.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Síndromes Mielodisplásicos
/
ARN Mensajero
/
Ácido Valproico
/
Apoptosis
/
Péptidos y Proteínas de Señalización Intercelular
/
Línea Celular Tumoral
/
Proliferación Celular
/
Proteína Reguladora de Apoptosis Similar a CASP8 y FADD
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Artículo
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