Genetic characterization of the N protein of subgroups A and B human respiratory syncytial viruses / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 115-117, 2009.
Artículo
en Chino
| WPRIM
| ID: wpr-332411
ABSTRACT
<p><b>OBJECTIVE</b>To clarify the genetic characteristics of N protein coding region of HRSV isolates from Beijing and GenBank downloaded sequences.</p><p><b>METHODS</b>Reverse transciption polymerase chain reaction (RT-PCR) was performed to amplify the N protein gene of 2 A and 2 B subgroups HRSV isolates from Beijing in the year 2004. The RT-PCR products were sequenced for N protein coding region. The sequences of N protein coding region of 4 Beijing isolates and those downloaded from GenBank were compared and analyzed.</p><p><b>RESULTS</b>The differences in number of nucleotide and deduced amino acid between 2 A Beijing 2004 isolates and prototype strain Long were 36-40 (3.1%-3.4%) and 4 (1.0%). The differences in number of nucleotide and deduced amino acid between 2 B Beijing 2004 isolates and prototype strain CH18537 were 17 (1.4%) and 1 (0.3%). The differences in number of nucleotide and deduced amino acid were 3-172 (0.25%-14.63%) and 0-18 (0-4.6%) respectively between 4 Beijing 2004 isolates and GenBank sequences.</p><p><b>CONCLUSION</b>N gene is the highly conservative gene in the HRSV genome. The variation between A and B subgroups were widely distributed in the entire gene of N protein, while the variation within the A or B subgroups HRSV was considerably lower.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Virología
/
Datos de Secuencia Molecular
/
Química
/
Clasificación
/
Homología de Secuencia de Aminoácido
/
Virus Sincitial Respiratorio Humano
/
Infecciones por Virus Sincitial Respiratorio
/
Proteínas de la Nucleocápside
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2009
Tipo del documento:
Artículo
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