Preparation and characterization of monoclonal antibodies to rabies virus CVS-11 strain / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To get the high specific and sensitive mononclonal antibodies against RV.</p><p><b>METHODS</b>The myeloma cell line SP2/0 fused with the spleen cell of 6 - 8 weeks old BALB/c mice immunized with the CVS-11 virus antigen. The hybridized fusing cells were chosen by indirect ELISA detection and the positive hybridizing cells were amplified through mouse abdomen injection and the mouse McAbs ascites was purified by Protein A Sepharose 4 Fast Flow (Pharmacia Company). The specificity and sensitivity of the McAbs was identified by indirect ELISA and indirect DFA test.</p><p><b>RESULTS</b>The cell fusion rate reachs 100% and the indirect ELISA results showed that the McAbs ascites titer were 1 x 10(4), 1 x 10(5), 1 x 10(4) and 1 x 10(5); The immunoglobulin G type McAbs show no cross reaction with other related viruses.</p><p><b>CONCLUSION</b>The high specific and sensitive mononclonal antibodies of RV can be used for rapid RV diagnosis.</p>