Effective inactivatian test of inactivated hepatitis A vaccine using integrated cell culture/strand-specific reverse transcriptase-polymerase chain reaction / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 488-491, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-332457
ABSTRACT
<p><b>OBJECTIVE</b>To establish an quick, sensitive and specific assay for effective inactivatian test of inactivated hepatitis A vaccine.</p><p><b>METHODS</b>effective inactivatian test of inactivated hepatitis A vaccine were carried out using integrated cell culture/strand-specific RT-PCR (ICC/strand-specific RT-PCR) assay compared with traditional ELISA and nest RT-PCR assay.</p><p><b>RESULTS</b>all the samples were infectious negative detecting by both ICC/ strand-specific RT-PCR and ELISA assay,while some samples appeared false positive detecting by nest RT-PCR.</p><p><b>CONCLUSION</b>ICC/strand-specific RT-PCR assay is a novel, rapid, sensitive and reliable method for effective inactivatian test of inactivated hepatitis A vaccine. Shorting detection period largely, this assay may be used as an alternative method for routine inactivated hepatitis A vaccines test.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cultivo de Virus
/
Biotecnología
/
Ensayo de Inmunoadsorción Enzimática
/
Vacunas Virales
/
Vacunas de Productos Inactivados
/
Células Cultivadas
/
Sensibilidad y Especificidad
/
Técnicas de Cultivo de Célula
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Vacunas contra la Hepatitis A
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS