Pathogenic and tumorigenic effect of 1,2-dimethylhydrazine in mouse colon and ovarian / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the pathogenic and tumorigenic effect of 1,2-dimethylhydrazine (DMH) on the colon and ovaries of mice.</p><p><b>METHODS</b>Sixty ICR female mice were randomly divided into groups A and B for intraperitoneal injection of DMH (20 mg/kg) and saline (control) once a week for 24 weeks, respectively. The mice were sacrificed at 12, 16, 20, 24, 28 and 32 weeks after the first DMH injection for pathological examination of the colon and ovaries.</p><p><b>RESULTS</b>In group A, colorectal adenomas were found in 7, colorectal adenocarcinomas in 5, and hemorrhagic lesions of the ovaries with chronic inflammatory in 21 mice. Choriocarcinoma in the ovaries were detected in one mouse at 28 weeks and in another at 32 weeks. No obvious pathological changes were found in group B following the injections.</p><p><b>CONCLUSION</b>Intraperitoneal injection of DMH may induce colon tumors and ovarian diseases in mice.</p>