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Lack of Effect of Dexamethasone on Growth of Orientia Tsutsugamushi Gilliam in Mouse L929 Cells
Yonsei Medical Journal ; : 624-629, 2011.
Artículo en Inglés | WPRIM | ID: wpr-33257
ABSTRACT

PURPOSE:

Previous studies and our own clinical experience suggest that concurrent corticosteroid treatment for severe rickettsial disease with multiorgan failure may improve the clinical course or reduce mortality. However, the use of corticosteroids as adjunctive treatment for rickettsial diseases is controversial. We attempted to determine the influences of corticosteroid on the growth of Orientia tsutsugamushi in vitro to justify and evaluate the clinical applicability of corticosteroid in rickettsial disease. MATERIALS AND

METHODS:

L929 cells were infected with Orientia tsutsugamushi Gilliam. Dexamethasone was added to the cells at final concentrations of 10(1) and 10(7) pg/mL. Cultures were incubated at 35degrees C and processed for flow cytometry on the 6th day after addition of dexamethasone.

RESULTS:

Observation on the 6th day after treatment with dexamethasone in infected cultures revealed that there was no difference in fluorescence intensity among the treatment wells. Treatment of the cells with dexamethasone at concentrations of 10(1) and 10(7) pg/mL showed no influence on the growth of Orientia tsutsugamushi.

CONCLUSION:

Our results to show that isolated corticosteroid does not enhance the replication of Orientia tsutsugamushi in vitro. Concurrent use of anti-inflammatory or immunosuppressive doses of corticosteroids in conjunction with antibiotics may not have detrimental effects on the course of scrub typhus.
Asunto(s)

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Orientia tsutsugamushi / Dexametasona / Tifus por Ácaros / Línea Celular / Interferón gamma / Proliferación Celular / Citometría de Flujo Límite: Animales Idioma: Inglés Revista: Yonsei Medical Journal Año: 2011 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Orientia tsutsugamushi / Dexametasona / Tifus por Ácaros / Línea Celular / Interferón gamma / Proliferación Celular / Citometría de Flujo Límite: Animales Idioma: Inglés Revista: Yonsei Medical Journal Año: 2011 Tipo del documento: Artículo